ABSTRACT
In order to study the effects of chronic malnutrition on lung surfactant, adult male Wistar rats reciving a standard diet (SD) were comparaed to animals submitted to food restriction (FR). SD rats given food and water and libitum and FR rats were allowed half (10g) of their usual food comsumption and water ad libitum, for 28 days. the evaluation of the pulmonary surfactant included pressure-volume curve with air inflation, pH and artherial blood gas measurements with rats breathing room air and 100 percent oxygen and determination of phospholipids in the lung washouts. In the pressure-volume curve, the volumes retained at 5 and 10 cmH2O of transpulmonary pressure (Tpt) and the Tpt of 40 percent of total lung capacity (TLC) were not significantly different between the two groups, showing that food restriction did not increase the surface forces. the increase in TLC/lung wet weight ratio in the FR group, probably was secondary to decrease in the elastic recoil forces in the lungs. The PaO2 did not show any significant difference between the groups. The arterial blood pH and PCO2 were also similar in both, SD and FR groups. Total phospholipid content in the lung washouts related to be weight was not signifiantly different in SD as compared with FR rats. Therefore, in this malnutrition model of rats, no alterations in pulmonary surfactant could be shown
Subject(s)
Rats , Animals , Male , Nutrition Disorders , Lung/metabolism , Pulmonary Surfactants/metabolism , Blood Gas Analysis , Body Weight , Disease Models, Animal , Lung Volume Measurements , Organ Size , Phospholipids/metabolism , Lung/blood supply , Rats, WistarABSTRACT
Os efeitos toxicos de sudoxicam, N-(2-tiazolil)-4-hidroxi-2-metil-2H-1,2-benzotiazina-3-carboxamida 1,1-dioxido, foram estudados em ratos Wistar, determinando suas toxicidades aguda e subaguda. A DL50, baseada na mortalidade ocorrida no periodo de 6 dias apos administracao oral de dose unica de sudoxicam,foi de 144,0 mg/kg. Doses diarias, por via oral, de 1 mg/kg e 5 mg/kg durante 30 dias foram bem toleradas pelos animais. Quanto ao ganho de peso corporal e consumo de racao, nao houve diferenca significante entre os animais tratados e os do grupo controle, nesse periodo experimental. O exame macroscopico do figado, baco e rins nao revelou nenhuma lesao nos orgaos. A atividade teratogenica de sudoxicam foi testada em embrioes de galinha, injetando-o no saco vitelino de ovos embrionados de galinhas Garrinson, no 8o. dia de incubacao. Observou-se maior letalidade com a utilizacao de doses elevadas. Entretanto, nao foi constatada nenhuma malformacao nas doses de 5 micrograma/ovo e 500 micrograma/ovo